India were without Ishant Sharma in the morning as the fast bowler was unfit to bowl after falling on his follow-through in Saturday's last session.
The injuries to Sachin Tendulkar and Laxman forced under-pressure India to change their batting order.
Tendulkar batted down the order after suffering an elbow injury on Saturday and Laxman made his appearance with a runner (Gambhir) after twisting his ankle during practice before the second day's play.
Sourav Ganguly (18) batted ahead of Tendulkar, but did not last long as he was adjudged leg-before off spinner Muttiah Muralitharan by umpire Mark Benson of England.
The batsman requested the official to review his decision under a new experimental rule which is on trial in the ongoing series.
The rule allows a batsman or fielding captain to request a review of any decision by referring it to the third official monitoring television replays.
Benson's initial decision turned out to be correct when he consulted TV umpire Billy Doc