The Cricket Association of Bengal (CAB), which runs the stadium, had asked the ICC to reconsider its controversial ruling on Friday.
But ICC chief executive Haroon Lorgat said CAB's request could not be accepted due to logistic issues.
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"The ICC cannot accommodate the request," Lorgat was quoted as saying by Cricinfo website. He said there were too many planning and ligistical issues that would need to be involved.
The game's governing body has also asked the Board of Control for Cricket in India (BCCI) to confirm an alternative venue for the tie by the end of this month.
The Chinnaswamy Stadium in Bangalore has now emerged as the front-runner to host the Pool B game.
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The ground is already set to host four World Cup matches, starting with England versus Ireland on March 2. Eden Gardens, w